Glycine betaine enhances growth of nitrogen-fixing bacteria Gluconacetobacter diazotrophicus PAL5 under saline stress conditions
Published in Current Microbiology, 2009
Recommended citation: Boniolo FS, Rodrigues RC, Delatorre EO, da Silveira MM, Flores VMQ, Berbert-Molina MA. Glycine Betaine Enhances Growth of Nitrogen-Fixing Bacteria Gluconacetobacter diazotrophicus PAL5 Under Saline Stress Conditions. Curr. Microbiol. 2009 Dec 18;59(6):593–9.
Abstract
In this study, the effect of glycine betaine as osmoprotectant compound for Gluconacetobacter diazotrophicus PAL5 was evaluated by kinetic growth parameters. Batch fermentation assays were performed employing media supplemented with different sodium chloride concentrations to simulate saline stress conditions. Salt concentrations of 50–300 mM led to decreased cell concentrations, while the maximum specific growth rates and cell productivities were reduced at concentrations above 100-mM NaCl. Salt inhibition was mainly observed in media with 200- and 300-mM NaCl, in which drastic changes in cell morphology were also noted. The addition of glycine betaine to the media showed to be efficient to counteract the salt inhibitory effect by increasing some fermentation parameters. However, the osmoprotectant was not able to revert the polymorphism promoted by higher salt concentrations.
